Simple. Fast. Elegant: RNA-seq library prep with integrated ribo-globin depletion
Innovative Solution for Transcriptomic Library Preparation
RNA-seq studies carried out using high-throughput sequencing of cDNA have provided tremendous insight into cellular transcripts studies on a large and more comprehensive scale. However, technical challenges such as laborious and lengthy workflows, affordability, compromised accuracy, read coverage biases, and limited transcript diversity have impeded implementation of the technology in many labs.
We introduce the sparQ RNA-Seq HMR Kit that integrates RNA fragmentation and depletion of abundant ribosomal and globin transcripts from multiple species (human, mouse, and rat) into a single step, and tube. The proprietary, highly optimized enzymes and streamlined workflow generates high quality directional transcriptome NGS libraries from either intact or degraded RNA samples in 5 hours.