Designed to generate full length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.
- For synthesis of single-stranded cDNA prior to amplification by PCR
- Features preassembled bulk reaction mixes to save time and minimise potential for pipetting errors
- Allows users to choose primers
Includes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18.
Informace o dodávce: Kit includes sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)6 Primer, NotI-(dT)18 Bifunctional Primer and instruction booklet.