These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells.
- Cylinders are tapered so bottom edge is wider than the top ensuring a good sealing surface
- Polystyrene cloning cylinders are supplied in sterile packs
- Three sizes: 4,7 mm Int. Ø×8 mm H, 6,4 mm Int. Ø×8 mm H and 9,5 mm Int. Ø×11 mm H
To isolate a clone, a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding a small volume of Trypsin or EDTA, the dish is incubated at 37 °C (98,6 °F) briefly until cells detach. Cells are then collected from inside the cylinder and transferred to another vessel for further growth.