Affinity chromatography resin, chelating nickel

Dodávateľ: G-Biosciences
786-408EA 4960.5 EUR
786-408 786-394 786-281 786-393 786-392 786-407
Affinity chromatography resin, chelating nickel
Média pro purifikaci proteinů
Nickel chelating resin is an immobilised metal affinity chromatography (IMAC) resin that was specifically designed to utilise Ni²⁺ for the purification of nickel binding proteins, including 6x histidine tagged proteins.

Nickel chelating resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent nickel ion with a capacity of 20 to 40 μmoles Ni²⁺/ml resin. The protein binding capacity is >50 mg protein per ml resin. We have demonstrated binding of >100 mg of a 50 kDa 6X His tagged proteins to a ml of resin. The spin columns are supplied with a resin bed volume of 0,2, 1 and 3 ml with total column volumes of 1, 8 and 22 ml respectively. Columns can be used as a spin format of gravity flow columns.
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