peqGOLD Taq DNA Polymerase is a highly processive 5'→3' polymerase with no 3'→5' exonuclease (proofreading) activity. At 72 °C and around pH 9 conditions, peqGOLD Taq reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds.
- Thermostable polymerase for standard PCR applications
- Also available as 'all inclusive' (with dNTPs) or as 'Hot Start'
peqGOLD Taq is supplied with Buffer Y for maximal yield and Buffer S for specificity, along with Enhancer Solution P which modifies the denaturing characteristics of DNA to improve amplification of difficult templates.
* One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid insoluble material in 30 minutes at 74 °C.
Informace o dodávce: Product includes Taq DNA (or Hot Start Taq DNA) Polymerase at a concentration of 5 units*/µl; Dilution Buffer; 10X Reaction Buffer Y: 200 mM Tris-HCl (pH 8,55), 160 mM (NH₄)₂S₄, 0,1 % Tween 20 and 20 mM MgCl₂; 10X Reaction Buffer S: 100 mM Tris-HCl (pH 8,8), 500 mM KCl, 0,1 % Tween 20 and 15 mM MgCl₂; Enhancer solution P; and 25 mM MgCl₂.