Pierce™ azide-labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.
- Bioorthogonal - the azido-sugar compounds do not interfere with endogenous cellular pathways and substitute for their naturally occurring analogues
- Reaction chemistry with phosphine compounds occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
- Azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency
- Azide tag can be targeted for detection, immobilization, conjugation or affinity purification depending on which phosphine-activated compound it is reacted with
These sugars are azide-derivatives of naturally occurring monosaccharides that cells use to glycosylate proteins using post-translational modification biochemical pathways. When supplied to cells, these compounds become incorporated by glycosylation events to effectively 'tag' glycoproteins with the azide group. The azide group then can be specifically targeted for detection or conjugation using alkyne-activated reagents. When used in combination with phosphine-activated fluorescent dyes, biotin reagents, and or other compounds, these azido-modified sugars facilitate the investigation of cellular pathways involving glycosylation.