The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.
- Requires less than 10 µg of antibody for a single IP experiment
- Eliminates antibody contamination
- Immobilise 100 to 200 µg of antibody to prepare ready-made IP affinity resin
- Spin columns eliminate resin loss and provide for more efficient separation of solutions
The primary benefits resulting from this method are the opportunity to use any species or subclass of purified antibody (not just types that bind to Protein A or G) and the ability to purify target protein without contamination by the antibody. The method also makes it possible to immunoprecipitate antigens from serum samples without co-purifying non-target immunoglobulins. The kit uses microcentrifuge spin cups to effectively wash and separate samples from the beaded agarose resin.
Informace o dodávce: Kit includes AminoLink™ Plus coupling resin, 2 ml; coupling buffer (20X), 25 ml; quenching buffer, 50 ml; wash solution, 50 ml; sodium cyanoborohydride solution (5M), 0,5 ml; IP lysis/wash buffer, 2×50 ml; conditioning buffer (100X), 5 ml; tris-buffered saline (20X), 25 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; control agarose resin, 2 ml; spin columns; and collection tubes.
