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Cobalt spin filter plates, 96 well, HisPur™

Dodávateľ: Thermo Scientific
Pierce
PIER90095EA 582.69 EUR
PIER90095
Cobalt spin filter plates, 96 well, HisPur™
Mikrodestičky Destičky filtrační
HisPur™ Cobalt spin plates are convenient, ready-to-use, pre-dispensed filter plates for the efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells. These spin plates are ideal for simultaneous processing of multiple small (1 mg/well binding capacity) samples.

  • Obtain more than 10 mg of pure His-tagged protein per ml of resin without optimising imidazole washing conditions
  • Cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
  • No metal contamination in eluted histidine-tagged protein sample
  • Purify proteins under native or denaturing conditions
  • Resin can be reused several times or discarded after single use

The spin plates contain a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins. This resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole).

Informace o dodávce: Includes two 96-well filter plates with each well containing 100 µl of resin bed (400 µl of 25% resin slurry), and six wash/collection plates.
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