Clelandovo činidlo ≥99.5%, white powder for electrophoresis
Dodávateľ: MP Biomedicals
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Varování
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DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.
- Electrophoresis Grade
- Half Life: 1.4( pH 8.5 at 20 °C)
- pH: 4.0 to 6.0 (0.1 M in H2O, 25 °C)
- Redox potential = -0.33 volts at pH 7
- Soluble in water (50 mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
- +4 °C
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Vzorec:
C₄H₁₀O₂S₂ Mw: 154,25 g/mol Teplota tavenia: 40…50 °C Skladovacia teplota: Refrigerator |
Číslo MDL:
MFCD00004877 Číslo CAS: 3483-12-3 OSN: 3335 ADR: 9,III |
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Výsledky špecifikačného testu
| Identity Test | Passes |
| Purity | ≥99.5% |
| FTIR | Conforms to Standard |
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