Everest columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Previously, the 300 Å C18 chemistries had carbon coverage in the 2,8 to 3,6 µmol/m² range. Everest C18 coverage is in excess of 4 µmol/m² and approximates the theoretical limit based on surface area. The increased shielding of the base silica increases column lifetime and reduces the amount of TFA required to shield the silica.
- Unique selectivity for hydrophilic and hydrophobic peptides
- High resolution for complex peptide digests
- Excellent sensitivity with little or no TFA in mobile phase
Vydac 238EV (C18) offers maximum surface coverage for the highest resolution of complex samples. It is recommended for the analysis of complex enzymatic digests (>12 proteins).
* Use with holder GCH10 and column coupler HI-081.
** Use with holder 80101/N and column coupler HI-081.