The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
- Packed in a glass bottle
- Store at ≤–70 °C
The sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB070). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
Informace o dodávce: The pET vectors are supplied as purified plasmid DNA (10 µg). It includes an induction control strain (supplied as a glycerol stock).