Tlačiť

WB blocking buffer kit, TrueBlack®

Dodávateľ: Biotium

23013-TEA 142.74 EUR
23013-T 23013.
WB blocking buffer kit, TrueBlack®
Pufry Pufry pro elektroforézu Pufry blokovací
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

  • Blocks non-specific protein binding, reducing background fluorescence over the entire membrane
  • Suppresses background caused by charged fluorescent dyes (performs better than BSA, gelatin, or casein)
  • Reduces antibody cross-reactivity, eliminating non-specific bands
  • Compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes
  • Can be used with antibodies labeled with all types of fluorophores spanning the visible and NIR spectrum
  • Contains non-mammalian blocking agents, for broad secondary antibody compatibility
  • TBS based buffer suitable for phosphoprotein detection

The buffers are designed to achieve optimal specificity and sensitivity by blocking background from the non-specific interaction between the fluorophore-labeled antibodies and the membrane. Non-specific signal in WB can arise from multiple sources, including antibody cross-reactivity with off-target proteins, non-specific antibody adsorption to the membrane, and membrane autofluorescence. Another potential cause of background that is not well-known is the effect of fluorescent dyes themselves on the specificity of labeled antibodies.

Next-generation fluorescent dyes like Alexa Fluor® or CF® dyes often carry multiple negative charges to improve dye solubility and brightness of conjugates. The extra charge carried by the dye can result in non-specific antibody binding and background fluorescence. The TrueBlack® WB Blocking Buffer Kit blocks background from multiple sources including charged dye conjugates. It is especially advantageous for phosphoprotein detection, significantly improving specificity compared to conventional blocking buffers.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Viac About VWR