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PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye

Dodávateľ: Biotium

40019.EA 266.96 EUR
40019.
PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye
PMA (Propidium monoazide)

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.


  • PMA (40013) is formulated as 20 mM in water to avoid the use of DMSO which might facilitate the dye entering into the live cell.
  • Abs = 464 nm (before photolysis)
  • Abs/Em = 510/610 nm (following photolysis and covalent attachment to DNA/RNA)
  • Dark red liquid
  • Store at -20°C and protect from light at all times


The PMA-modified dsDNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR. A bacterial sample can be first pre-treated with PMA under light to inactivate dsDNA from dead cells. DNA is then extracted and analyzed by PCR. PMA is also available in lyophilized format (40013).


Since Biotium first developed PMA dye, there have been hundreds of publications on the use of the dye in many sample types including dozens of bacterial strains, biofilms, yeast, fungi, viruses, and eukaryotic cells. It has been used in such applications as food and water safety and environmental testing, and has been used in conjunction with qPCR, NextGen Sequencing (NGS), Sanger sequencing, and Loop-mediated Isothermal Amplification (LAMP). Check the Downloads section to view the PMA&PMaxx™ Reference List and the PMA&PMAxx™ Validated Bacterial Strains List.


For photoactivation of PMA dye, we recommend our PMA-Lite™ LED Photolysis Device for microcentrifuge tubes, or the Glo-Plate Blue™ LED Illuminator for larger tubes or 96-well plates.

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