FLUO 3/AM ≥95% (by HPLC) fluorescent indicator, for calcium determination

Dodávateľ: Biotium

50014.EA 371.87 EUR
50014. 50016. 50013.
FLUO 3/AM ≥95% (by HPLC) fluorescent indicator, for calcium determination

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

  • Abs/Em (after hydrolysis): 506/526 nm (low or high [Ca2 ] )
  • Extinction Coefficient (after hydrolysis): 86,000 M-1 cm-1 (506 nm)
  • Kd (Ca2 ) (after hydrolysis): 450 nM
  • Cell permeability: Membrane permeant
  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution

Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. See the product information sheet for more information.

Vzorec: C₅₁H₅₀Cl₂N₂O₂₃
Skladovacia teplota: Freezer
Číslo CAS: 121714-22-5

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