Fura 2-AM ≥98% (by HPLC) fluorescent indicator, for calcium determination

Dodávateľ: Biotium

50033-1EA 380.82 EUR
50033-1 50034. 50033.
Fura 2-AM ≥98% (by HPLC) fluorescent indicator, for calcium determination
Fura 2-AM

Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.

  • Kd: 145 nM (22°C in pH 7.2 buffer) (after hydrolysis)
  • λEx/λEm (no Ca2+) = 363/512 nm; λEx/λEm (high Ca2+) = 335/505 nm (after hydrolysis)
  • ε (363 nm, no Ca2+) = 27,000 M-1cm-1; ε (335 nm, high Ca2+) = 35,000 M-1cm-1 (after hydrolysis)
  • Cell permeability: Membrane permeant
  • Light yellow solid soluble in DMSO
  • Store at -20°C and protect from light, especially in solution

Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once it enters cells.

Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.

Vzorec: C₄₄H₄₇N₃O₂₄
Skladovacia teplota: Freezer
Číslo MDL: MFCD00036976
Číslo CAS: 108964-32-5

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