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T4 DNA polymerase

Dodávateľ: Fermentas (Thermo Scientific)
EP0061EA 61.92 EUR
EP0061 EP0062
T4 DNA polymerase
Činidla pro nukleové kyseliny Nucleic Acid Modification and Labelling
T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

  • Stronger 3' - 5' exonuclease activity
  • Active in PCR, RT and T4 DNA Ligase buffers

Applications include blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs, blunting of PCR products with 3'-dA overhangs, synthesis of labeled DNA probes by the replacement reaction, oligonucleotide-directed site-specific mutagenesis, ligation-independent cloning of PCR products.

Storage buffer: 20 mM potassium phosphate (pH 7,5), 200 mM KCl, 2 mM DTT, and 50% (v/v) glycerol.

Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 37 °C.

Informace o dodávce: Supplied with 0,35 ml of 5X reaction buffer.

Pozor: For research use only. Not for use in diagnostic procedures.
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