illustra™ RT-PCR is commonly used to amplify cDNA by combining first-strand cDNA synthesis of an RNA template with PCR amplification. Typically the reaction is performed in two steps. A first-strand cDNA reaction is performed, the resulting cDNA is then transferred to another tube containing Taq DNA polymerase and PCR buffer where the reaction mixture is subjected to multiple cycles of denaturation, annealing and elongation, resulting in the exponential amplification of the target cDNA. Ready-To-Go™ RT-PCR beads simplify this process to a single tube, single reaction procedure.
- Pre-dispensed, single dose, ambient-temperature-stable beads ensure greater reproducibility between reactions, minimise pipetting steps and reduce the potential for pipetting errors and contamination
- Optimised as a one-tube, one-step RT-PCR reaction for both cDNA synthesis and PCR using only one buffer and primer set - no need to open the tube or change conditions between steps
- Each lot of RT-PCR beads is function tested for its ability to generate highly specific PCR products to ensure lot-to-lot reproducibility
Ready-To-Go™ RT-PCR beads have been optimised for RT-PCR. Each room-temperature-stable bead contains M-MuLV Reverse Transcriptase, RNase inhibitor, buffer, nucleotides and Taq DNA polymerase. The only additional reagents required are water, template RNA, and primers.
The Ready-To-Go™ bead format significantly reduces the number of pipetting steps, thereby increasing reproducibility of the RT-PCR technique and minimising risk of contamination and RNA degradation. Ready-To-Go™ RT-PCR beads are provided in either thin walled 0,5 or 0,2 ml tubes compatible with most thermocyclers. The 0,2 ml tubes come assembled in a 96-well (8×12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight or individual 0,2 ml tubes.
Informace o dodávce: Each package of Ready-To-Go™ RT-PCR beads contains RT-PCR beads, control reactions and pd(N)₆ and oligo(dT) cDNA primers.