Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.
- Effective for suppressing proteolytic activity in detergent-based cell lysis reagents
- Convenient packaging - single-use packaging is ideal for small scale or infrequent use; 5 ml size available for increased use
- EDTA-free formulation - metalloproteases may be inhibited by addition of the optional EDTA stock solution; EDTA-free formulation inhibits proteolytic activity (except metalloproteases) in applications where EDTA may affect protein stability and function; omit EDTA to prevent interference with subsequent assays or purification methods such as immobilised metal chelate affinity chromatography (IMAC)
- Stable refrigerated stock solution - saves valuable freezer space; no waiting for tablets to completely dissolve; easily dispense the correct amount of inhibitor