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Protease inhibitor cocktail, Halt

Dodávateľ: Thermo Scientific
Pierce
PIER78439EA 611.66 EUR
PIER78439 PIER78425 PIER87786 PIER78438 PIER78430 PIER78429 PIER87785 PIER78415 PIER78410 PIER78437
Protease inhibitor cocktail, Halt
Doplňky a přísady médií z buněčných kultur
Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

  • Effective for suppressing proteolytic activity in detergent-based cell lysis reagents
  • Convenient packaging - single-use packaging is ideal for small scale or infrequent use; 5 ml size available for increased use
  • EDTA-free formulation - metalloproteases may be inhibited by addition of the optional EDTA stock solution; EDTA-free formulation inhibits proteolytic activity (except metalloproteases) in applications where EDTA may affect protein stability and function; omit EDTA to prevent interference with subsequent assays or purification methods such as immobilised metal chelate affinity chromatography (IMAC)
  • Stable refrigerated stock solution - saves valuable freezer space; no waiting for tablets to completely dissolve; easily dispense the correct amount of inhibitor
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