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cDNA synthesis, qScript™ Ultra SuperMix

Dodávateľ: Quantabio
qScript µltra SuperMix
High Range Input Linearity. First-strand cDNA synthesis reactions were carried out using increasing quantities of total human RNA input, followed<br />by analysis of a portion of the products by beta-actin qPCR
Low input reaction sensitivity. First-strand cDNA synthesis reactions were assembled and reacted with 2 pg total human RNA, then a portion (15%)<br />of the reaction was used for qPCR analysis
Viral RNA cDNA synthesis in the presence of carrier RNA
Inhibitor resistance across wide range of sample types. Standard, randomer-primed first-stand cDNA synthesis reactions were carried out using 50 ng<br />total human RNA in the presence of several common reaction inhibitors
Balanced coverage of long transcripts. qPCR primer assays were<br />designed at several intervals along the length of the 6.3 kb MAP4 mRNA<br />transcript. First strand cDNA synthesis using qScript µltra SuperMix was<br />performed with total RNA input levels of 100 ng, 10 ng, 1 ng and 100 pg
Comparison of protocol length for first-strand cDNA synthesis kits. Incorporating the highly rapid and processive qScript µltra reverse transcriptase,<br />the qScript µltra SuperMix requires 10 minutes for first-strand cDNA synthesis over a broad range of input RNA quantities
qScript µltra SuperMix
High Range Input Linearity. First-strand cDNA synthesis reactions were carried out using increasing quantities of total human RNA input, followed<br />by analysis of a portion of the products by beta-actin qPCR
Low input reaction sensitivity. First-strand cDNA synthesis reactions were assembled and reacted with 2 pg total human RNA, then a portion (15%)<br />of the reaction was used for qPCR analysis
Viral RNA cDNA synthesis in the presence of carrier RNA
Inhibitor resistance across wide range of sample types. Standard, randomer-primed first-stand cDNA synthesis reactions were carried out using 50 ng<br />total human RNA in the presence of several common reaction inhibitors
Balanced coverage of long transcripts. qPCR primer assays were<br />designed at several intervals along the length of the 6.3 kb MAP4 mRNA<br />transcript. First strand cDNA synthesis using qScript µltra SuperMix was<br />performed with total RNA input levels of 100 ng, 10 ng, 1 ng and 100 pg
Comparison of protocol length for first-strand cDNA synthesis kits. Incorporating the highly rapid and processive qScript µltra reverse transcriptase,<br />the qScript µltra SuperMix requires 10 minutes for first-strand cDNA synthesis over a broad range of input RNA quantities
733-2905EA 1937.17 EUR
733-2905 733-2904 733-2903
cDNA synthesis, qScript™ Ultra SuperMix
Činidla pro nukleové kyseliny RT-PCR and cDNA Reagents
Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

  • Enhanced thermostability – Engineered for RT activity up to 65 °C, overcoming challenging secondary structures
  • Superior speed – 10 minute reaction time with a total protocol time of 13 minutes
  • Maximum yield and sensitivity – Wide dynamic range with no loss in cDNA abundance linearity from total RNA 2,5 μg to 1 pg
  • Ultimate inhibitor resistance – Overcome a wide array of PCR inhibitors (salt, heparin, melanin, etc.)
  • Balanced coverage of long transcripts – Unbiased representation of the transcriptome in cDNA product
  • Ease of use – Single tube mix with all required components for cDNA synthesis except RNA template

qScript Ultra SuperMix is a highly stabilized, efficient and easy-to-use single tube master mix for the synthesis of first-strand cDNA to reverse transcribe RNA to cDNA. A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase that was engineered for improved thermostability, velocity, processivity, and resistance to many common inhibitors. qScript Ultra SuperMix contains all required components for first-strand cDNA synthesis except RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR procedures.

qScript Ultra SuperMix is engineered for RT activity up to 65 °C, with an optimal reaction temperature of 55 °C. The reaction time is 10 minutes with a total protocol time of 13 minutes. This rapidly reduces time to result while working through challenging secondary RNA structures.

qScript Ultra SuperMix provides maximum yields across a wide variety of RNA input amounts, optimised for 2,5 μg to 1 pg total RNA input with even as little at 100 fg total RNA possible. This product demonstrates fine level discrimination and linear conversion of RNA to cDNA with a high copy reference gene at high input RNA amounts, which further emphasises accurate and critical expression levels. High efficiency and precision of RNA to cDNA conversion creates optimal cDNA yields for downstream use.

qScript Ultra SuperMix demonstrates low-copy sensitivity, linear dynamic range and higher yields of viral RNA in the presence of high levels of carrier RNA. This allows for low RNA input quantities to be detected with consistent yield and reduced dropout. The Quantabio PerfeCTa SYBR Green SuperMix and FastMix were both used for qPCR amplification.

qScript Ultra SuperMix can readily reverse transcribe RNA while overcoming common inhibitors found in blood, skin, plants, and RNA extraction carryover chemicals. The robust, engineered qScript Ultra reverse transcriptase allows for tolerance to many common reaction inhibitors. qScript Ultra SuperMix consistently produces high yields of cDNA even in the presence of these challenging inhibitors. The Quantabio PerfeCTa SYBR Green FastMix, low ROX was used for qPCR amplification.

qScript Ultra SuperMix can readily reverse transcribe long RNA transcripts in an unbiased manner, maintaining equivalent representation in the qPCR amplification curves throughout the length of the transcript. First strand cDNA synthesis using qScript Ultra SuperMix was performed with total RNA input levels of 100 ng, 10 ng, 1 ng and 100 pg. The Quantabio PerfeCTa SYBR Green FastMix was used for qPCR amplification.
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