Tetrazolium blue chloride (BTC) is a colourless tetrazolium compound that upon reduction forms a colored diformazan.
The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. Useful in assay of reducing sugars. Also used in the preparation of DNA cation complexes and immuno-adsorbents for anti-DNA antibodies.
Soluble in hot water (50 mg/10 ml), methanol (50 mg/ml - clear yellow to yellow green solution), ethanol, chloroform; slightly soluble in water; insoluble in ethyl acetate, acetone, ether.