Corning® PuraMatrix™ Peptide Hydrogel is a synthetic matrix that is used to create defined three-dimensional (3D) microenvironments for a variety of cell culture experiments. To achieve optimal cell growth and differentiation, it is necessary to determine the appropriate mixture of this material and bioactive molecules (for example, growth factors, extracellular matrix (ECM) proteins, and/or other molecules). Corning® PuraMatrix™ Peptide Hydrogel consists of standard amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel that exhibits a nanometer scale fibrous structure (average pore size 50 to 200 nm). Hydrogel promotes the differentiation of hepatocyte progenitor cells, rat pheochromocytoma cells (PC12), and hippocampal neurons, and supports the attachment of a variety of primary (for example, neuronal, fibroblast, keratinocyte) and transformed (for example, MG-63, SH-SY5Y, HEK293, NIH3T3) cell types. Potential applications include stem cell proliferation, tumour cell migration/invasion, angiogenesis assays, and in vivo analyses of tissue regeneration.
- Hydrogel is a highly defined material that promotes cell attachment and which is readily formed in a culture dish, plate, or cell culture insert
- Easily mixed with cells and/or bioactive molecules (such as growth factors) prior to gelation, and is injectable for in vivo studies in animals
- As Hydrogel is transparent, samples can be readily visualised using standard staining methodologies and microscopy
- Established protocols are available for 3D cell encapsulation cultures; surface plating of adherent cells on microporous membrane inserts and microplates; cell recovery for sub-culturing of biochemical analyses; and in vivo injection