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Maxima™ SYBR® Green qPCR Master Mix (2X), with ROX

Dodávateľ: Fermentas (Thermo Scientific)
K0253EA 3654.47 EUR
K0253 K0251 K0252
Maxima™ SYBR® Green qPCR Master Mix (2X), with ROX
Činidla pro nukleové kyseliny qPCR/RT-qPCR Enzymes and Kits
The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

  • Specificity – eliminates non specific amplification and formation of primer dimers
  • Sensitivity – detects low copy number targets
  • ROX solution provided in a separate tube
  • Wide linear range – accurate quantification across 9 orders of magnitude
  • Can be used on most real time PCR instruments
  • Ready to use

Informace o dodávce: Supplied with 50 µM ROX solution and nuclease-free water.
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