CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
- High efficiency: Complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
- Fast: Reaction is completed in 15 minutes at 37 °C
- Stable at −20 °C for up to 2 years
Informace o dodávce: M.SssI is supplied with an optimised 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.