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CpG methyltransferase

Dodávateľ: Thermo Fisher Scientific
EM0821EA 235.8 EUR
EM0821
CpG methyltransferase
Enzymy
CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

  • High efficiency: Complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
  • Fast: Reaction is completed in 15 minutes at 37 °C
  • Stable at −20 °C for up to 2 years

Informace o dodávce: M.SssI is supplied with an optimised 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.
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