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Maxima™ SYBR Green/Fluorescein qPCR Master Mix (2X)

Dodávateľ: Fermentas (Thermo Scientific)
K0241EA 250.2 EUR
K0241 K0243 K0242
Maxima™ SYBR Green/Fluorescein qPCR Master Mix (2X)
Činidla pro nukleové kyseliny qPCR/RT-qPCR Enzymes and Kits
The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

  • Specificity – eliminates non specific amplification and formation of primer dimers
  • Sensitivity – detects low copy number targets
  • Wide linear range – accurate quantification across 9 orders of magnitude
  • Can be used on most real time PCR instruments
  • Ready to use

* UDG is not included in the master mix and must be purchased separately.
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