Maxima™ SYBR Green/Fluorescein qPCR Master Mix (2X)Dodávateľ: Fermentas (Thermo Scientific)
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
- Specificity – eliminates non specific amplification and formation of primer dimers
- Sensitivity – detects low copy number targets
- Wide linear range – accurate quantification across 9 orders of magnitude
- Can be used on most real time PCR instruments
- Ready to use