GeneJET™ Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. GeneJET™ Plasmid Maxiprep Kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
- Efficient: High yields up to 750 µg of high quality plasmid DNA from 250 ml of bacterial culture
- High concentration of DNA purified: >400 ng/µl
- Convenient: Spin column format, centrifuge or vacuum protocols
- Fast: Procedure takes 60 minutes, considerably faster than using gravity flow columns
Informace o dodávce: Each kit includes resuspension solution, lysis solution, neutralisation solution, endotoxin binding reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A solution, elution buffer (10 mM Tris-HCl, pH 8,5), GeneJET™ Maxi purification columns pre-assembled with collection tubes (50 ml), and collection tubes (50 ml).