DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl2 and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
For applications that require PCR product analysis by absorbance or fluorescence excitation, the colourless DreamTaq PCR Master Mix (2X) is recommended.